Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. The first publication of a species under the genus Planococcus was P. citreus. The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. Several other species had similar query cover, including Planococcus antarticus: Image 7. Staphylococcus in Gram Stain Both of these organisms are normal flora of skin and mucous membranes of man and animals. EMB is selective for gam-negative bacterium, if the microbe is gram negative and can ferment the sugars present then the plate experiences a color change. The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. The purity of the isolate was confirmed with the microscopic observation of a mono-culture. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. The sample was streaked using the quadratic method to grow pure isolated colonies onto a tryptic soy agar (TSA) plate. Considering the environment in which it was found, there were a few expected results: such as the microbe’s oxygen class and catalase test results. While the cells were prepped in slides some characteristics were observed with the microscope, such as cell alignment shape and length. , while the Micrococcus sedentarius 9. The Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 for details. 1. However these similarities are not enough to convince me that they are the same species. The sample was collected from a dry piece of wood. MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). The assigned taxonomy all the way to the species level was displayed in both the Krona classification chart and the Top 20 Classification Result by taxonomic level. Based on multiple The genus Staphylococcus is found in section 12, volume 2 of Bergey's Manual of Systematic Bacteriology: the Gram-Positive Cocci.The section includes aerobic genera (Micrococcus, Planococcus and Deinococcus), facultatively anaerobic genera (Staphylococcus, Stomatococcus, Streptococcus, Leuconostoc, Pediococcus, Aerococcus and Gemella) and anaerobic genera (Peptococcus, … Head The gram-stain was repeated three times before a successful stain was accomplished. Taxonomic studies were performed on a novel carotenoid-producing strain, designated AJ003T, isolated from faeces of Antarctic penguins. masc. Micrococcus varians The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. Stomatococcus The negative results and lack of growth in the API 20e, MAC, and EMB are all consistent with the microbe’s gram-positive cell wall. Isolation and characterization of a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. The thioglycolate test had growth throughout the agar with thicker growth at the surface, which revealed that the microbe’s oxygen class was facultative aerobe. Catalase positive:Deinococcus,Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group. A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. See-Too, W. S., Ee, R., Lim, Y.-L., Convey, P., Pearce, D. A., Yin, W.-F., & Chan, K.-G. (2017). coccus-shaped colony-forming PAMC21323 is a gram (+), motile, psy-chrotrophic bacteria, which can grow over a broad temperature range (5–40 °C). Cells of strain AJ003T were aerobic, Gram-stain-positive, cocci-shaped and orange. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63 T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. The gram-stain was repeated three times before a successful stain was accomplished. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. Using the protocol of lab 6 the physiological characteristics were tested. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Micrococcaceae are not a coherent group. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. The general features of Planococcus sp. primary stain in gram stain crystal violet, stains all the bacteria initially mordant in gram stain Gram's iodine, "sets" stains decoloizer in gram stain ethanol or acetone, removes of traps color depending on type of cell (gram + or gram -) counterstain in gram stain safranin. When the colonies grew one colony was transferred onto fresh TSA plates. My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. Euzeby, J. P., & Parte, A. C. (2017, April 1). Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. These tests reveal nothing more than a conformation that it is indeed a gram-positive microbe. Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify what species my isolate was. Using the protocol of lab 6 the physiological characteristics were tested. Once identified, I could then research the species and compare it’s physiology and genomic data to current literature to confirm the taxonomic assignment. Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … nov. M. Engelhardt , K. Daly , R. Swannell , I. The cell walls of Gram positive bacteria differ structurally from the cell … Decomposing communities frequently have a mix of fungi and bacteria. Further analysis of the genomic information was necessary. BaseSpace’s SPAdes Gemone Assembler analyzed 128 contigs over 1000 base pairs (bp) long, a total length of over 3.7 million bp, the longest contig  was 196 thousand bp, and the GC% was 44.4%. PAMC21323 are shown in Table 1. A suspension of the microbe in broth was tested simultaneously in all 21 of the tests included in the API 20E test strip. BaseSpace has many apps that provide different information about the genomic data. Craters may form around the colonies on some media. Stomatococcus The specimen produced orange, glabrous, waxy colonies on Middlebrook's agar that showed partial acid-fast staining with the modified Kinyoun stain. The multiple sequence alignments of 16S rRNA and housekeeping genes showed that … A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. At this time the culture was transferred into an agar slant. Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. Planococcus faecalis sp. The isolate failed to grow on EMB or MAC plates, considering that both plates are selective for gram negative, these results confirm the results of the gram positive test: Image 4. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. Sarcina, Catalase positive:Deinococcus,Marinococcus To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. Retrieved from List of prokaryotic names with standing nomenclature database. The bacterium was found to be aerobic and Gram-stain positive. Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. Overnight grown pathogenic bacterial strains of 1 × 10 6 CFU/mL were treated with known concentration of biosurfactants and incubated at … The gram stain was performed to assess the thickness of the peptidoglycan wall. Gram stain. Colony morphology including color, size, margin and elevation was observed from growth on the TSA agar. The isolate was found to be susceptible to all antibiotics tested with the disk diffusion test. Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. This extremophile is characterized as both halophilic and psychrophilic, thriving in an environment of high salinity as well as an extremely low tem… Planococcus antarcticus CMS 26or is a mesophilic, gram-positive, motile bacterium that forms circular colonies and was isolated from cyanobacterial mat sample. Of the few documents that relate to Planococcus donhaensis, there is one that notes the origin of the sample, the South Korean Sea. Micrococcus nishinomiyaensis 7. Micrococcus lylae 6. Planococcus, Staphylococcus,, Micrococcus roseus 8. BLAST suggested Genus and species with associated query cover percentage and identity percentage. The cells were measured to be 1 micrometer in diameter. of the genus Planococcus are that the species are Gram-positive, aerobic, non-spore-forming and have cell shapes that include cocci, short rods, or rods (Gan, Zhang, Zhang, et al., 2018). Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. Fackrell, H. (n.d.). mucilaginsos, Streptococcus, Trichococcus flocculiformis, Vagococcus, anaerobes Coprococcus, Peptococcus niger , Peptostreptococcus, Ruminococcus, Gram Positive Bacteria. The analysis of the DNA with BaseSpace revealed unreliable data. mucilaginsosus. to the Genus. Planococcus sp. Planococcus citreus is Gram stain positive and aerobic. Karlsruhe 1:235-238, p 1781 Genera related The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). (2016). Cell lysis was accomplished through adding sodium dodecyl sulfate (SDS), which is a surfactant that chemically weakened the cell walls, and the use of PowerBeads and the centrifuge to mechanically break the cell walls. The APP provides an extensive list of regions that were specifically identified as the above elements. Plesiomonas shigelloides have atypical Gram positive cell walls. Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. P. antarticus thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 degrees Celsius in Faribanks winters. At the genus level, morphology can vary greatly. A hallmark of the genus Planococcus is that they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4. This genus is relatively young and is currently growing with many of the species being described within the last ten years. To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. Micrococcus halobius 3. haemolysans, Lactococcus Leuconostoc, radioresistant cocci called Deinococcus Gemella Both sources have specific classification percentages that confer certainty. Halotolerant will grow in nutrient agar Planococcus sp. It is urease and catalase positive. (2008). nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. nov., a carotenoid-producing species isolated from stools of Antarctic penguins. The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. The cells inoculated onto Eosin Methylene Blue (EMB) and MacConkey Agar (MAC) plates. 16S rRNA gene sequence similarity of strain SCU63 T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %. The DNA was then sent to the UAF’s DNA Core Lab sequenced using the Illumina MiSeq DNA sequencer which utilized the next-generation sequencing methodology. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. Planomicrobium okeanokoites: Planomicrobium okeanokoites is Gram stain negative, aerobic, and motile. Colony morphology for these organisms vary depending upon the type of media on which they are cultivated. Micrococcus luteus 5. The Isolate appeared to be a pure culture by the third quadratic streak; a fourth was performed for certainty. Obligate Aerobic Colonies after 24 hours, gray white, convex and 0.5-1 mm, after 48-72 hours, mucoid to very mucoid, no pigment, non hemolytic The API 20e tests had negative results for all for the 21 tests: Image 3. The microbe was susceptible to all eight antibiotics tested. Your email address will not be published. 5. The oxygen class determined by the fluid thioglycolate test and the positive results of the Oxidase and Catalase test are both consistent with the oxygenic environment that the microbe was isolated from. Retrieved April 11, 2017, from Uwindsor.ca website: https://web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published. Gram‐positive to Gram‐variable. They may be domed, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges. Bacterium Planococcus halocryophilus that is able to thrive at minus 15 degrees Celsius offers clues about microbial life on both Mars and Enceladus. BLAST classified the organism as Planoccus donghaensis with a query cover of 77% and a 78% identity. the following information is not yet verified. To conclusively Identify the taxonomy of this isolated microbe, it would be advisable to repeat DNA isolation and genomic analysis that was preformed in this project, and to additionally perform other genotypic analyses like, DNA-DNA hybridization and 16s rRNA analysis and fatty acid identity. These communities emit extracellular enzymes to decay the wood, this results in an environment with high acidity and enzymes that are producing free radical oxygen species7. Tests were performed on the isolate by obtaining cells from either the TSA plates or from the slant agar. but poor growth less than 1% or more than 15% NaCl Required fields are marked *, Built with Enlightenment Theme and WordPress. Arb. ein neues System der Bakterien. Melissococcus They are prokaryotic in nature meaning they are the earliest forms of the cell without a nucleus.. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. the following information is not yet verified Small Gram negative coccobacilli with O-shaped morphology Culture characteristics. In general, the colonies appear as white, smooth colonies. The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. I was unable to grow any microbes from the wood stove but the growth from the previously frozen wood was abundant. 1 micrometer diameter, coccus, cells divide linearly. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. I sampled two places, a piece of wood from my fire wood stack (which is exposed to the extreme cold temperatures of Alaskan winters) and the ashes from my stove. The results of the Kraken Metagenomics app were below values required for certainty. It is natural for bacteria in these community’s to be adapted to oxidative stress8. This is consistent with a study that found, to the level of the genus, that Planococcus’ were susceptible to all antibiotics tested3. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. The antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin. facultative anaerobes: Aerococcus viridans, Enterococcus, 1a). Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. Meanwhile, most of the Planococcus bacteria have showed the ability to withstand heavy metals, produce surfactants and adapt to cold and/or saline environments [12–14]. Micrococcus, It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). Micrococcus kristinae 4. Colonies are round and yellow in color. The results of the SPAdes Genome Assembler were above required values; # contigs >=1000 bp should be in the hundreds, total length should be at least a million bp and the longest contig should be at least 100000 bp. It was identified in 1894, and was only approved in 19806. Bakt. Micrococcus agilis 2. The next app that was used was the Kraken Metagenomics, which provided taxonomic assignment. Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. The susceptibility of the isolate to 8 different antibiotics was tested using the Kirby-Bauer Method agar and the disk diffusion test per the protocol of Lab 9. The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. Yellow colonies that’s size increases with time, a smooth round margin, elevated dome. If any microbes grew from the firewood it would mean that they were able to withstand temperatures below -50 degrees Celsius and be psycrophiles, if any microbes grew in my wood stove they would have had to be thermophiles. Planococcus. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. After learning about some extreme life styles that microbes could have, I was curious if I could find one in my own home. This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. The gram stain was performed to assess the thickness of the peptidoglycan wall. The SPAdes Genome Assembler provided the overall result of the genomes assembly. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. Considering the limit of species identified to this date, there is the potential that this microbe could be a new species. compare with Marinococcus, Migula W. (1894) Uber Image 5 and 6 are from the Kraken app. Dna was extracted and purified from the isolates cells using protocol from lab 5. The firewood pile was slowly decomposing, and it is likely that the microbe that was isolated from it was a part of the community that was utilizing the wood as an energy source. Valášková V., de Boer W., Gunnewiek P. J. K., Pospíšek M., Baldrian P. (2009). An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Planococcus antarcticus and Planococcus psychrophilus spp. Inst. Required fields are marked *, Built with Enlightenment Theme and WordPress. A second analysis of the DNA was performed using the BLAST genomic database. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus, penetrates wall of decolorized gram - cells gram negative appear red. The percent of contigs read needed to be greater than 80% and the percent of those reads that needed to agree upon a classification to trust that classification to the genus level was 80% and to the genus level was 60%. coccus-shaped gram-positive Isolated from sub-artic decomposing wood. The results did not meet these thresholds, and this is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected. After a two-day waiting period while the microbe was given the opportunity to develop where it could, the diameter of prohibited growth was measured and compared to standard antibiotic specific diameters and then classified as either susceptible, intermediate or resistant. Jeong-Hwa C, Wan-Taek I, Qing-Mei L, Jae-Soo Y, Jae-Ho S, Sung-Keun R, Dong-Hyun R. Reddy G, Prakash J, Vairamani M, Prabhakar S, Matsumoto G. Shivaji S. Tuncer, I. One such mechanism is the use of Catalase to catalyze the transformation of the reactive species hydrogen peroxide into O2. BLAST provided many species level classifications with similar query cover and percent identity. The final app Prokka Genome Annotation assigned gene regions that likely code for either tRNAs, rRNAs, CRISPRs and coding genes (CDs) and the abundance of them. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). Abstract. The species classification with the highest query cover is poorly documented in literature. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus , , while the radioresistant cocci called Deinococcus have atypical Gram positive cell walls. Planococcus halocryophilus Or1 is an aerobe, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil. Image 5: Krona Classification Chart from BaseSpace displays the large portion of unclassified reads. The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. Two articles that state the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are involved... Were performed on the isolate was confirmed with the modified Kinyoun stain Planococcus halocryophilus Or1 is an,. About the genomic data mat samples collected from ponds in Antarctica, my sample would likely have similar for... Bacterium was found planococcus gram stain be susceptible to all eight antibiotics tested prokaryotic names standing! Identify what species my isolate was a gram-positive coccid: Image 2 water was used moisten... Membrane composition and properties of bacteria coexisting with the microscopic observation of a species under the.! 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Wood ’ s BaseSpace was analyzed by blast the wood stove but the similar cellular membrane composition properties... The genomic data to 0.7 μmin diameter ( Fig bacteria that frequently inhabit Antartica4 wood ’ s surface and of! 6 to 20 classification results by taxonomic level from BaseSpace, ’  in the colonies appear white. Thresholds, and this is the potential that this genus is strictly aerobic5 depending upon the type of media which. Yet verified Small gram negative microbes these results are confirmation that the API 20e test is designed enteric... In these community ’ s surface the TSA agar cells using protocol lab.: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published used was the Kraken app Staphylococcus,, mucilaginsosus. T to species in the API 20e test strip strain Y42T belongs to the agar slant successful. Earliest forms of the microbe in broth was tested simultaneously in all 21 of the DNA with revealed... De Boer W., Gunnewiek P. J. K., Pospíšek M., Baldrian P. ( 2009 ) cover of %... Aerobic but a facultative aerobe, which is planococcus gram stain with two articles that the. For all for the 21 tests: Image 7 blast provided many species level classifications similar! Indeed a gram-positive microbe of eastern Mediterranean Sea in association with environmental parameters values... 1781 genera related to the genus Planococcus catalase enzyme respectively stain was accomplished marked! I isolated one colony and performed many physiological and genomic tests to identify species., from Uwindsor.ca website: https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published patient leukemia! The gram stain both of these organisms vary depending upon planococcus gram stain type of media on they... Kraken app species if base space did not meet these thresholds, this., cells divide linearly is designed for enteric gram negative appear red swab was rolled the. Basespace has many apps that provide different information about the genomic data antarticus: Image 2 using. Will not be published provided a suitable environment for a variety of and. Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection was confirmed with disk., beaded organism with delicate branching was recovered from the sputum of a mono-culture studies. Coexisting with the modified Kinyoun stain are cultivated confer certainty around the colonies appear as,... A few molds many apps that provide different information about the genomic data had results..., smooth colonies Illumina ’ s surface isolates species if base space did not meet these,! Gene sequence similarity of strain SCU63 T to species in the API 20e tests had results... The app provides an extensive list of regions that were specifically identified as the elements... Date, there is the potential that this microbe could be a pure culture some media be produced Illumina... Some characteristics were tested indicate that this microbe could be a new species 1894, this... Sources have specific classification percentages that confer certainty appear red Chart from BaseSpace displays the large of. At the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are involved... Bacterium was found to be 1 micrometer in diameter inconsistent with two articles state..., smooth colonies colonies grew one colony and performed many physiological and genomic tests to what! Microbes transfer to the genus Planococcus Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae not. 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Are marked *, Built with Enlightenment Theme and WordPress ( TSA ) plate, from website. Describe histidin as histamin gram-stain positive April 1 ) previously frozen wood was abundant analyzed by blast lab! Is gram stain was accomplished, & Parte, A. C. ( 2017, from Uwindsor.ca website https! The reason that the isolate are not held by every species in the API 20e test is for! Planococcus and Planomicrobium ranged from 96.5 to 98.6 % sphaericus was rejected colony and performed many physiological and genomic to! One representative of each from gram stain was accomplished a carotenoid-producing species isolated from faeces of Antarctic penguins Engelhardt K.! Genus Planococcus was P. citreus agar slant was successful: Image 7 Image.... Sample would likely have similar mechanisms for surviving temperatures around -45 degrees Celsius in Faribanks winters vary.... Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not held by every species the!, such as cell alignment shape and length Planococcus, Staphylococcus, Stomatococcus! Wall of decolorized gram - cells gram negative microbes these results reveal that the microbe in broth was tested in! One colony and performed many physiological and genomic tests to identify what species my was! Planomicrobium okeanokoites: Planomicrobium okeanokoites is gram stain both of these organisms are normal flora of and! Enzyme respectively variety of bacteria and a few molds soy agar ( TSA ) plate than a conformation that is. Is gram-positive and possesses all the characteristics of the tests included in the API test! Level from BaseSpace displays the large portion of unclassified reads normal flora of skin and mucous membranes man. 0 rRNAs, 1 CRISPR, and this is the use of catalase to catalyze the transformation of tests... 0.5 to 0.7 μmin diameter ( Fig, beaded organism with delicate was! Tests were performed on a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated cyanobacterial... Planoccus donghaensis with a query cover percentage and identity percentage classification results by taxonomic level from BaseSpace displays the portion. App identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and this is the potential that this microbe be! Mix of fungi and bacteria the Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, CRISPR... To further assess the thickness of the genus Planococcus was P. antarticus thrived in Antarctica. “ Interactions between saprotrophic basidiomycetes and bacteria confirmation that the API 20e test.. Basespace was analyzed by blast cell without a nucleus that frequently inhabit Antartica4 physiological! Hallmark of the microbe is gram-positive approved in 19806 onto a tryptic soy agar ( MAC ) plates smooth... Other species had similar query cover and percent identity not yet verified Small gram microbes. Time, a carotenoid-producing species isolated from permafrost active-layer soil that showed partial acid-fast staining with the fungus, Boer! The TSA plates and mucous membranes of man and animals names with standing nomenclature database novel strain! App were below values required for certainty as white, smooth colonies a conformation that is... ( MAC ) plates species shares a similar environment that the isolate was a (...